ISO 16187:2013 pdf download.Footwear and footwear components – Test method to assess antibacterial activity.
ISO 16187 specifies quantitative test methods to evaluate the antibacterial activity of footwear and components.
ISO 16187 is applicable to all types of footwear and components employing non- diffusing antibacterial treatments.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods ISO 19952, Footwear — Vocabulary
3 Terms and definitions
For the purposes of this document, the terms and definitions given in ISO 19952 and the following apply.
3.1
antibacterial activity
efficacy of a material or finish used to prevent or mitigate the growth of bacteria, to reduce the number of bacteria or to kill bacteria
3.2
control sample
material identical to the test material but without antibacterial treatment
4 Safety
Handling of microorganisms which are potentially hazardous requires a high degree of technical competence and can be subject to current national legislation and regulations. Only personnel trained in microbiological techniques should carry out such tests. Codes of practice for disinfection, sterilization and personal hygiene shall be strictly observed.
5 Apparatus and materials
5.1 General
Standard laboratory equipment and the following.
5.2 Biological safety cabinet.
5.3 Incubator, capable of maintaining a temperature ot (37 ± 2) C.
5.4 Autoclave.
5.5 Humidity chamber, capable of maintaining a temperature of(37 ± 2) °C and a relative humidity not less than 90 %.
5.6 Ultraviolet lamp.
5.7 Wide mouth jars, with cap, 100 ml, capable of being used with an autoclave (5.4).
5.8 Cover film that does not affect bacterial growth or absorb water, which can be made of either
polyethylene, polypropylene or polyester [poly (ethylene terephthalate)]. Film that is 0,05 mm to
0,10 mm thick is recommended. For example, disposal bag suitable for use with an autoclave (5.4).
5.9 Vortex mixer.
5.10 Dimensional shaker, two dimensional or three dimensional, capable of adjusting to 50 rpm.
5.11 Shaking incubator, capable of maintaining a temperature of (37 ± 2) °C and a rotational
frequency of (120 ± 10) rpm.
6 Reagents and culture medium
6.1 Principle
The preparation and test shall be freshly prepared in order to ensure the culture quality. NOTE This can be done according to ISO/TS 11133-1, ISO/TS 11133-2, or according to national standards or regulations.
Reagents used in tests shall be of analytical grade and/or suited for microbiological purposes. Use only water Grade 3 according to ISO 3696.
6.2 Nutrient broth (NB)
6.2.1 Composition
Beef extract, 3,0 g.
Peptone, 5,0 g.ISO 16187 pdf download.